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OncoGenomics (Molecular Cytogenetics)


The OncoGenomics (Molecular Cytogenetics) Laboratory provides a diagnostic service for malignant haematological disorders and carries out research in cancer genomics. The laboratory works closely with the internationally respected bone marrow transplant unit and other specialist units in the hospital.


Tests offered:

  • Karyotyping by G-banding
  • FISH screening (single test or disease target panels)
  • Microarray CGH (chromosome microarray analysis/ molecular karyotyping)
  • Next Generation Sequencing (Myeloid malignancies target panel)
  • Genetic screening of CD138+ cells isolates

A record of the sample details should accompany all samples submitted for cytogenetic analysis. See the Cytogenomics request form.


Samples

For optimal results, samples for conventional cytogenetic tests and FISH analysis should be collected in preservative-free heparin or in sterile containers provided by the laboratory. Call on +44(0)20 7307 9400 ext 3711 (office) / 3612 (lab) to obtain ‘Cytogenetic collection containers’.

Samples for DNA-based molecular karyotyping and/or next generation sequencing (NGS) analysis should consist of fresh or frozen cells (bone marrow aspirate, lymph node biopsy, trephine, needle biopsy, pleural effusion or ascites) or 5–8μg total genomic DNA.

Cytogenetic analysis requires living cells. Please ensure the sample reaches the Laboratory within 24 hours of donation. Multiple myeloma samples should arrive by 3.00pm on the day of sampling whenever possible. Samples should not be frozen, i.e.; below 1°C or exposed to excess heat, i.e. above 38°C. If there is a delay in transit please store the sample at 4°C (in a refrigerator).

Factors that significantly affect the performance of the examination or interpretation of results:

  • Clotted samples
  • Aged Samples (>48 hours)
  • Haemolysed samples
  • Under-filled or over-filled transport medium tubes.

 

Reporting times

Urgent FISH results such as PML-RARA may be available within 4 hours of receipt of sample. Prior agreement with the lab is required for all urgent tests. Tests for query acute leukaemia (e.g. AML, ALL, PCL, CML at presentation, transforming CML and acute phase of NHL) can be urgently processed with results available within 10 calendar days. The reporting time for the remaining samples is normally within 21 calendar days. Molecular karyotyping and NGS analyses are carried out according to current requirements of 21 calendar days.

 

Requesting additional tests

Additional tests can be requested at anytime via UCLH/SIHMDS HiLIS or by emailing the lab directly via oncogenomics@hslpathology.com.